Product Description
Product Description
TypeChemiluminescence immunoassay analyzerBrandHappycareModelHC-B016LanguageEnglishTechnical parameters:Sample form: 96 holes or barsLinear range: 100—2000K relative light unitsSensitivity: TSH analyzing sensitivity ≤0.01uIU/mlInterference between adjacent holes: Less than 1×10-6Repeatability: CV<2%Detecting speed: 96 holes in 3 minutes (96 holes sample test)Time of measurement: 0.1-6s/holeBarcode identification: Barcode is used for the parameters of all matching reagent in order to facilitate using, handling andstorage by the end user, and to reduce artificial error as well. Communication port: RS232 serial port or USB high speed port.Operation environment: 110-250VAC,50-60HzSize: 644mmX440mmX278mmWeight: 15KG1,PurposeThe system and its reagent sets are for ultra-microanalysis, quantitative determination and qualitative analysis of the chemicalcompositions of virus antigen, antibody, hormone, peptide, tumor protein, metabolite and medicine in serum and other body fluid.2,Applications & Principles Technical Theory:Enzyme-labeled Chemiluminescence Immunoassay Technology Test Procedure:1) Sandwich ELISA:Solid Phase Chemiluminescence Enzyme Immunoassay, taking micro plate as its carries, follows the sandwichmethodology. The methodology uses 2 specific antibodies which recognize discrete sites on the unknown molecule. The first antibodyis precoated onto the wells of the micro plate. It captures antigen in the blood serum after being added into the sample. Theother antibody, connected with HRP, is allowed to bind to the antigen. Thus, the immune compounds of antibody -antigen- labeledantibody come into being on the surface of the micro plate. After unbound antigen and HRP-antigen-antibody are removed with a washby solution, chromogenic substrate solution is added to determine the relative luminescence intensity. According to the relativeluminescence intensity of the known antigen concentration of the calibration materials, standard curve is drawn and regressionline is available via dual-logarithm mathematical model. Then, the concentration of antigen present in the unknown sample can becalculated.2)Competitive ELISA: Solid Phase Chemiluminescence Enzyme Immunoassay, taking micro plate as its carries, follows the competitivemethodology. Antigen is coated in advance onto the wells of the micro plate. After the test sample is added, free antigen from thetest sample and the HRP-labeled antigen compete for the immobilized antibody-binding sites on the wells. Thus, the immunecompounds consisting of antibody-antigen and antibody-HRP- labeled antigen come into being on the surface of the micro plate.After unbound antigen and HRP-antigen are washed off by solution, pre-set chromogenic substrate solution is added to determine therelative luminescence intensity. The more the free antigen in the sample, the less the amount of conjugated HRP-antigen. Accordingto the known concentration of the calibration materials and its related luminescence intensity, dosage-reaction curve is drawn andregression line is available after the fitting process. Then, the concentration of antigen present in the unknown sample can becalculated.3,Specifications1) Hardware System-X-axis & Y-axis Plane Drive SystemComposed of X-axis sensor, Y-axis sensor, X-axis stepping motor control, X-axis drive mechanism and 96-well micro plate pallet.Its function is to cause the plane movement of the 96-well micro plate so as to make each sample well exactly orient to test site. 2) Hardware System-System ControlComposed of Y axis motor control, low ripple power supply, three-dimensional drive control, photon counting and operation, as wellas communication interface. It is in charge of movement control, data processing and data transmission.3) Hardware System- Photon ReadComposed of such cells transferring, as optical fiber movement, photon accelerating, high-voltage dispatching and signalpretreatment. Its function is to realize the optical isolating, photon transferring, light-to-electric conversion andsignal-figure processing. 4,Operation ConditionAmbient Temperature:15~35℃ Relative Humidity:≤85%Power Supply: 220V±22V;50Hz±1Hz5,Hardware System-Embedded Computer System(Only for ECLIA-IIM Series )Made up of industry control embedded system. Its function is to implement display, data input, instrument control, fittingoperation, database, report forms printing, etc.
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