WEST TUNE QPCR Accurate 96 Real-time Quantitative PCR---PCR West Tune QPCR Accurate 96 is based on global vision of product design concepts and manufacturing processes. It creatively combines Fresnel lens optical signal acquisition technology, time-resolved signal separation technology and unique temperature control technology. And it reaches international advanced level in sensitivity, multi-color crosstalk, temperature uniformity and accuracy. It supports the application of all common QPCR detection modes.
| Temperature control system |
| Sample capacity | 0.2ml PCR tubes×96, 8×12 PCR plate or 96 well plate ×1 |
| Reaction volume | 10-50 μl |
| Thermal cycle technology | Peltier |
| Max. Heating/Cooling rate | 6.0° C/s |
| Heating temperature range | 4 - 100 °C |
| Temperature accuracy | ± 0.2°C |
| Temperature uniformity | ±0.2ºC @60ºC , ±0.2ºC @95ºC |
| Temperature gradient setting range | 30-100°C |
| Temperature gradient difference setting range | 1 - 36 °C |
| Detection system |
| Excitation light source | 4/6 monochrome high efficiency LEDs |
| Detection device | PMT |
| Detection mode | Time-resolved signal separating technology |
| Excitation/detection wavelength range | 455-650nm/510-715nm |
| Fluorescent channels | 4/6 channels |
| Supported dye | FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ |
| Sensitivity | Single copy gene |
| Resolution | 1.33 folds copy number difference can be distinguished in single-plex qPCR |
| Dynamic range | 10 orders of magnitude copies |
| Analysis mode |
| Absolute quantification, Melting curve analysis, Endpoint fluorescence analysis |
| Data output |
| Original file, Excel data file, results include run settings, graph and tabular data results |
