PW501

Product Details

Product Description

Product Description
Brand:YOKOGAWA
Type:PW501
Origin:the United States
Warranty: 365 days
Colour: new/used
Shipping method: Courier delivery
Products are widely used in metallurgy, petroleum, glass, aluminum manufacturing, petrochemical industry, coal mine, papermaking, printing, textile printing and dyeing, mechanical, electronic manufacturing, automobile manufacturing, plastic machinery, electric power, water conservancy, water treatment/environmental protection, boiler heating, energy, power transmission and distribution and so on.
Spare parts spare parts, the main DCS control system of PLC system and the robot system spare parts,
Brand advantage: Allen Bradley, BentlyNevada, ABB, Emerson Ovation, Honeywell DCS, Rockwell ICS Triplex, FOXBORO, Schneider PLC, GE Fanuc, Motorola, HIMA, TRICONEX, Prosoft etc. Various kinds of imported industrial parts
Appendix ssDNA Purification & Isolated DNA in DNA/RNA Shield cDNA clean-up The DCC® kit can be used to effectively clean and concentrate cDNA (> 500 nt) following reverse transcription (RT) in the presence/absence of fluorescent dyes. Unincorporated free nucleotides and fluorescent derivatives are efficiently removed using the DCC®, and the recovered cDNA may be used directly for microarray analysis, second-strand cDNA synthesis, or indirect labeling with a fluorescent dye such as NHS ester Cy3 or Cy5. Note: For clean-up of short cDNAs or ESTs (≥ 16 nt), we recommend the Oligo Clean & Concentrator (Cat. Nos. D4060, D4061). Hydrolysis 1. Add 10 µl 0.5 M EDTA and 10 µl 1 N NaOH to 50 µl of RT reaction. The volumes of EDTA and NaOH should be scaled proportionally depending on the starting volume of the RT reaction. 2. Incubate at 65°C for 15 minutes. Clean-up 1. Add 490 µl (7 volumes) of DNA Binding Buffer to the hydrolysis reaction above. Mix well. Neutralization (pH) following RNA hydrolysis is not necessary as the DNA Binding Buffer will effectively neutralize the NaOH added to the reaction. 2. Continue with Step 2 of the Protocol on page 6. M13 phage ssDNA purification
1. Centrifuge phage-infected bacterial culture at 8,000 x g for 1 minute. 2. Transfer 100 µl of phage-containing supernatant to a 1.5 ml microcentrifuge tube and add 700 µl (7 volumes) of DNA Binding Buffer. Mix briefly by vortexing. Increased supernatant volumes may be processed by proportionally increasing the amount of DNA Binding Buffer added to the sample. 3. Continue with Step 2 of the Protocol on page 6.Isolated DNA stored in DNA/RNA Shield For previously isolated/purified DNA stored in DNA/RNA Shield, use the following protocol to recover ultra-pure DNA, ready for downstream applications. 1. If frozen, thaw samples1 at room temperature (20-30°C). 2. Add an equal volume of ethanol (95-100%) to the sample and mix well. 3. Continue with clean-up protocol, page 6, step 2.

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