Product Details
Product Description
Product Description
Brand:PROSOFT
Type:PLX32-EIP-SIE
Origin:the United States
Warranty: 365 days
Colour: new/used
Shipping method: Courier delivery
Products are widely used in metallurgy, petroleum, glass, aluminum manufacturing, petrochemical industry, coal mine, papermaking, printing, textile printing and dyeing, mechanical, electronic manufacturing, automobile manufacturing, plastic machinery, electric power, water conservancy, water treatment/environmental protection, boiler heating, energy, power transmission and distribution and so on.
Spare parts spare parts, the main DCS control system of PLC system and the robot system spare parts,
Brand advantage: Allen Bradley, BentlyNevada, ABB, Emerson Ovation, Honeywell DCS, Rockwell ICS Triplex, FOXBORO, Schneider PLC, GE Fanuc, Motorola, HIMA, TRICONEX, Prosoft etc. Various kinds of imported industrial parts
Troubleshooting Low Recovery • Improperly Prepared/Stored DNA Wash Buffer Make sure ethanol has been added to the DNA Wash Buffer concentrate. Cap the bottle tightly to prevent evaporation over time. • Addition of DNA Elution Buffer Add elution buffer directly to the column matrix and not to the walls of the column. Elution buffer requires contact with the matrix for at least 1 minute for large DNA ≥ 10 kb. • Incomplete Elution 1. DNA elution is dependent on pH, temperature, and time. For large genomic DNA (≥ 50 kb), apply heated elution buffer (60-70 °C) and incubate for several minutes prior to elution. 2. Sequential elutions may be performed for quantitatively higher recovery but lower final DNA concentration. This is recommended for DNA ≥ 10 kb. Low A260/A230 Ratios • Column Tip Contaminated When removing the column from the collection tube, be careful that the tip of the column does not come into contact with the flowthrough. Trace amounts of salt from the flowthrough can contaminate a sample resulting in low A260/A230 ratios. Ethanol contamination from the flowthrough can also interfere with DNA elution. Zymo-Spin™ columns are designed for complete elution with no buffer retention or carryover (see below).
Following Clean-up with the DCC®, Multiple Bands Appear in an Agarose Gel • Acidification of DNA Loading Dye Most loading dyes do not contain EDTA and will acidify (pH ≤ 4) over time due to some microbial growth. This low pH is enough to cause DNA degradation. Therefore, if water is used to elute the DNA, 6X Loading Dye containing 1 mM EDTA is recommended.
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